How to cultivate oyster mushroom

Cultivation of Pleurotus ostreatus strains

1. Pleurotus ostreatus strain formula

Potato 200g, sugar 20g, agar 20g, water 1000ml, magnesium sulfate 3g and vitamin 2g. Accurately weigh according to the formula. Peel and slice the potatoes first, put them in 1000 ml water and cook with slow fire for about 20 minutes. Then filter, add water and heat. Firstly, agar, sugar, magnesium sulfate and vitamins are boiled until they are all dissolved. Measure whether there is enough 1000 ml with a cup. If not, make it up with boiling water. Put it into test tubes while it is hot, each tube contains 12ml, and 1000ml can hold 80- 100 test tubes. When repackaging, use a long funnel, and don't let the culture medium touch the test tube mouth, so as not to get wet by the cotton plug and pollute the miscellaneous bacteria. Cotton plugs should be made of ordinary cotton, and inserted into test tubes should be moderately elastic. Then every 10 test tube is tied into small bundles with leather sleeves, and every 5 small tubes are tied into large bundles. Wrap the tampon in kraft paper, put it in an autoclave with a pressure of 1.5 kg, sterilize for 40 minutes, and cease fire. When the pressure gauge returns to zero, vent and open the jar. Take out the test tube and put it on the table. There are two kinds of inclined test tubes: the inclined length of the original mother seed test tube should be 1/3 tube length, and the inclined length of the production mother seed test tube should be 1/2 tube length. After cooling and curing, take some test tubes and put them in a constant temperature box, and culture them in blank at 27-30 degrees for 65438. Inoculation should be carried out under strict aseptic conditions-629 special disinfectant for edible fungi, aerosol disinfection box, electronic inoculation device and so on can be used. After inoculation, it is placed in a 25-degree incubator for 7- 10 days, and when the test tube is full, it can be used to transfer secondary seeds.

Second, the secondary stock production of Pleurotus ostreatus

Formula 1: sawdust 80kg, corn flour 25kg, clotrimazole 1 kg, sugar 1 kg, magnesium sulfate 5kg, potassium dihydrogen phosphate 3kg, gypsum 1 kg, calcium carbonate 1 kg. The water content is 65%.

Formula 2: 80kg of corncob, 0/5kg of rice chaff/kloc-0, 0kg of aconite/kloc-0, 0/kg of clotrimazole, 0/kg of sugar/kloc-0, 5kg of magnesium sulfate, 2kg of potassium dihydrogen phosphate, 3kg of urea and 2kg of lime. The water content is 65%.

Formula 3: sawdust 40kg, corncob 40kg, bean cake powder 1 0kg, corn powder 1 0kg, sugar 1 kg, kemeiling1kg, magnesium sulfate 5kg, mushroom powder15g and potassium dihydrogen 3kg. Water content 65%

Formula 4: corn 100 kg, potassium dihydrogen phosphate 0. 5 kg, magnesium sulfate 0. 5 kg, sugar 1 kg, calcium carbonate 2 kg, 629 disinfectant 1 bottle.

Mixing: firstly, dry mix the main ingredients with Confucius or corn flour, gypsum and lime evenly. Then other drugs are dissolved in water to form a concentrated stock solution. Then the stock solution is evenly added into water and mixed into the material. Stir evenly and keep the water content at 65%, that is, hold a handful of materials with your hands after stirring, and there are water drops between your fingers but they don't fall. It is best to use Yatai brand bagging machine to mix materials, which is labor-saving and uniform, and can also pack various specifications of bacterial bags, with one machine for multiple purposes.

Bottle label: each bottle can hold 2.5 taels of dry materials, and the bottle weight after filling wet materials should be 1. 1 kg, which proves that the filling quantity and compactness should be moderate. Punching holes in the middle of the material is convenient for the rapid growth of hyphae. Immediately after bottling, clean the bottle body, cover it with propylene plastic, cover it with leather cover, and autoclave it with 1.5 kg for more than 2 hours. After sterilization, the temperature of the bottle is lowered below 30℃ and moved into the inoculation box. Must be strictly disinfected before inoculation-use 629 disinfectant or aerosol disinfection box or electronic inoculator. 5-8 bottles of secondary seeds can be transferred to the primary production mother seeds, and immediately put into an incubator at a temperature of 25-27 degrees for culture. If there is no incubator, it can be cultured at 25 degrees. Check it every 3-5 days and pick out unhealthy and impure ones in time. The bottle can be filled in about 25 days, and it will be consolidated for 5 days before it can be transferred to the third grade. The second seed can be packed in 12*24 propylene bags, because cans are not easy to buy, and plastic bags are used for large-scale seed production.

Key points of secondary seed production:

The materials should be dry without deterioration, the added raw materials must be accurate, and sterilization must be achieved to kill all microorganisms. Inoculation must be carried out under absolutely aseptic conditions, and the bottle must be covered with polypropylene plastic, because propylene plastic is more transparent than glass, and even miscellaneous bacteria the size of a small grain of rice can be clearly observed.

The formula of the third-grade seed can refer to the second-grade formula and production method completely, but the nutrient ratio of the culture medium should be close to that of the cultivation material, so that the mycelium can have a more adaptive process.

Third, the formula of cultivation materials

1, cottonseed hull 100 kg, lime 4 kg, magnesium sulfate 4 kg, kemeiling 1 2, urea 0.5 kg, potassium dihydrogen 3 kg, corn flour 10 kg, with water content of 65%.

2. cottonseed hull 100 kg, lime 1 0.5 kg, gypsum 2 kg, clotrimazole 1 kg and potassium permanganate1kg.

3. cottonseed hull 100 kg, quicklime 3 kg, phosphate fertilizer 1.5 kg, urea 2 kg, magnesium sulfate 3 kg, clotrimazole 1 kg, calcium carbonate 1 kg.

4. corncob 100 kg, corn flour 25 kg, potassium permanganate 1 kg, kemeiling 1 kg, potassium dihydrogen phosphate 32 kg, urea 32 kg, compound fertilizer 32 kg, magnesium sulfate 42 kg, calcium carbonate 1 kg, lime 4 kg, and mushrooms are rich in elements.

5. Corn cob 100 kg, Fuzi 15 kg, rice bran 20 kg, Kemeiling 2 kg, urea 5 kg, potassium dihydrogen phosphate 3 kg, magnesium sulfate 5 kg and lime.