How to do in vivo imaging experiment of mice?

In vivo imaging experiment of mice is an important method to study biological processes and disease development in animal models. Common in vivo imaging techniques include fluorescence imaging, bioluminescence imaging and single photon/multiphoton emission computed tomography (SPECT/CT).

General steps of imaging experiment in mice:

Preparation of experimental animals: Select appropriate strains of mice to ensure that they meet the experimental requirements, and make necessary domestication and environmental adaptation.

Labeling fluorescent probes or bioluminescent markers: according to the research needs, select appropriate fluorescent probes or bioluminescent markers to label experimental mice.

Preparation before imaging: Before imaging, mice need to be properly prepared, such as cleaning hair and fixing posture, to ensure the accuracy of imaging results.

Imaging operation: according to the selected in vivo imaging technology, the labeled mice are placed in imaging equipment for imaging. In the process of imaging, it is necessary to control environmental factors, such as temperature and humidity, to ensure the imaging quality and animal welfare.

Data analysis and interpretation: after obtaining the imaging data, analyze and interpret the data, and evaluate and interpret the imaging results in combination with experimental design and research problems.

When conducting in vivo imaging experiments of mice, we must strictly abide by the ethical norms of experimental animals and relevant laws and regulations to ensure the protection of animal welfare and rights. At the same time, it is necessary to choose the appropriate in-vivo imaging technology and operation method according to the specific research purpose and experimental conditions in order to obtain reliable experimental results. It is best to consult professionals before the experiment to ensure the scientificity and feasibility of the experiment.