The use of fluorescence microscope

Ultraviolet light is used as the light source to irradiate the detected object to make it emit fluorescence, and then the shape and position of the object are observed under the microscope. Fluorescence microscope is used to study the absorption, transportation, distribution and location of chemical substances in cells.

Some substances in cells, such as chlorophyll, will fluoresce after being irradiated by ultraviolet rays; Other substances themselves cannot emit fluorescence, but they can emit fluorescence after being dyed with fluorescent dyes or fluorescent antibodies and irradiated with ultraviolet rays. Fluorescence microscope is one of the tools for qualitative and quantitative study of these substances.

There are the following differences between fluorescence microscope and ordinary microscope:

1. The lighting mode is usually interrupted, that is, the light source is projected on the sample through the objective lens;

2. The light source is ultraviolet light with shorter wavelength and higher resolution than ordinary microscope;

There are two special filters, the one in front of the light source is used to filter out visible light, and the one between the eyepiece and the objective lens is used to filter out ultraviolet light to protect people's eyes.