How to look at bacteria under an optical microscope (1600 times)? Give an experimental example.

It stands to reason that bacteria can be seen under the optical microscope of 1600, and onion epidermal cells can be seen under the optical microscope of 600. So the key is operation or experimental design. Generally speaking, attention is paid to the light source and production. The resolution limit of optical microscope is 20 nm. Plant bacteria and fungi are micron-scale.

When you zoom in 400 times (10x, 40x), you can hardly see the bacteria, but it is only the size of a needle tip, just like a dot.

Generally, the magnification is still 1000 times (10x, 100x). At this time, the bacteria can still be seen clearly, and they can also be seen clearly after special flagella dyeing. You need to use an oil mirror to enlarge 1000 times.

Extended data:

The classical optical microscope is only a combination of optical elements and precision mechanical elements. It uses the human eye as a receiver to observe the enlarged image. Later, a photographic device was added to the microscope, and photosensitive film was used as the receiver for recording and storage.

In modern times, photoelectric elements, TV cameras and charge couplers are widely used as receivers of microscopes, and form a complete image information acquisition and processing system with microcomputers.

Curved optical lenses made of glass or other transparent materials can magnify objects, and optical microscopes use this principle to magnify tiny objects to the size that can be observed by human eyes. Modern optical microscope usually adopts two-stage magnification, which is completed by objective lens and eyepiece respectively. The observed object is located in front of the objective lens.

After the first amplification by the objective lens, it becomes an inverted real image, and then the real image becomes a virtual image after the second amplification by the eyepiece, which is what the human eye sees. The total magnification of a microscope is the product of the objective magnification and the eyepiece magnification. Magnification refers to the magnification of linear dimensions, not the area ratio.

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