Traditional Culture Encyclopedia - Photography major - Introduction to microphotography

Introduction to microphotography

Photographic methods commonly used in microphotography, teaching and sci-tech movies. That is, the microscope is connected with the photographic device, so that the image of the object observed through the microscope is recorded on the photosensitive film, thus obtaining the lens picture. It is a technical means to reveal the microscopic world invisible to the naked eye. Microphotography can record the activities of bacterial reproduction and growth, so that people can really understand various microscopic phenomena.

In order to obtain high-resolution microscopic images, apochromatic objective lens must be used to photograph with special eyepiece or flat eyepiece. The illumination modes of microphotography are divided into transmission illumination, incident illumination (vertical illumination) and light-dark field illumination. At the same time, we should also pay attention to the selection of various color filters.

Microphotography is to use an optical imaging system consisting of an objective lens and an eyepiece of a microscope as the lens of a camera to shoot specimens that are generally invisible to the naked eye. This kind of "enlarged video recording" of tiny objects can directly provide convenience for teaching and scientific research. According to the structure of the microscope, when the observed specimen is placed slightly outside the front focus of the objective lens, an enlarged inverted image will be formed at the inner side of the front focus of the eyepiece near the focus. At this time, the magnified inverted virtual image can be seen through the eyepiece (which acts like an ordinary magnifying glass).

If the imaging position of the objective lens is adjusted (the specimen can be properly far away from the objective lens or the eyepiece can be raised, that is, the distance between the eyepiece and the objective lens can be increased, so that the middle imaging is between one focal length and two focal lengths of the eyepiece), the image formed by the objective lens is outside the front focal length of the eyepiece, and this image is enlarged by the eyepiece, so that an upright real image can be obtained on the other side of the eyepiece. When the light source is strong enough, this image can make the negative film or photographic paper sensitive, or make the CCD of digital camera or video camera photoelectrical.