The photos obtained by microphotography should be as real as possible, and the characteristics of the samples taken should be restored. How to ensure this as much as possible?

Photographs obtained by microphotography should be as realistic as possible to restore the characteristics of the collected samples, as follows:

Set shooting parameters correctly. If you want to compare the fluorescence intensities of different samples, you should make sure to set the same parameters when shooting each sample. First of all, we can have a rough look at the fluorescence intensity of several samples to be compared.

Determine an appropriate parameter to avoid overexposure or overall darkness caused by the parameters of the first sample to the subsequent samples. If there is a big difference in fluorescence intensity between different samples due to improper dyeing treatment, it is suggested to re-dye and re-image.

Choose the appropriate magnification and resolution, and choose the objective lens with magnification according to the specific observation scene. Generally speaking, the imaging field of view of low power mirror is larger; The imaging field of view of high power mirror is small, but the resolution is high.

At the same time, it should be noted that when shooting with an objective lens with higher magnification, the required laser intensity is higher, and photobleaching and phototoxicity should be prevented. In addition, if you want to take a large mosaic with a high-power lens, you need to consider the time cost.

Microscopic action

1. Microscope is used to enlarge the image of tiny objects. Generally used in biology, medicine and microscopic particle observation.

2. Using the movement of the inching stage, measure the length with the reticle of the whole eyepiece.

3. Measure the angle with the rotating stage and the cursor differential angle dial at the lower end of the eyepiece, so that one end of the measured angle is aligned with the reticle and coincides with it, and then the other end coincides with it.