Brief introduction of autoradiography

Directory 1 pinyin 2 English reference 3 notes 1 pinyin z sh xi n sh y ng f.

2 English reference autoradiography

Note Autoradiography is also called autoradiography. It is a cytochemical and histochemical method, which uses photographic film or emulsion to observe the uptake of radioactive substances in organisms, so as to determine the distribution, transfer and metabolism of substances in organisms. That is to say, compressed specimens and sliced specimens or living bodies that have ingested specific radioactive substances are placed in a dark room and closely contacted with photographic emulsion. The part exposed by the radiation emitted by radioactive substances ingested in the living body shows black silver particles after development. According to different observation levels, it can be divided into macroscopic autoradiography (visible autoradiography), microscopic autoradiography (autoradiography displayed by optical microscope) and ultramicroscopic autoradiography (autoradiography displayed by electron microscope). Macroscopic autoradiography is not only used to check the distribution of substances in tissue slices, but also used to determine trace substances together with paper chromatography and electrophoresis. The radionuclides used are 13 1I, 32P, 35S, 90Sr, 59Fe, 45Ca and14c; Autoradiography by naked eye. Microscopic and ultramicro autoradiography methods mainly use 14C and 3H.