What are the applications of microplate readers in agriculture?

What are the applications of microplate readers in agriculture?

Isotope tracing Isotope tracing has been widely used in agricultural production and research. It is mainly used to study the photosynthesis of crops, material transport in the body, fertilizer utilization, agricultural residues and nutritional metabolism of livestock, etc., so as to correctly formulate measures for crop cultivation management and reasonable and effective application of fertilizers and agriculture, and for the feeding and management of livestock. etc. to provide scientific basis. What are the applications of EDTA in agriculture?

In 1999, the annual consumption of EDTA in Europe was 35,000 tons and in the United States was 50,000 tons. Its important uses are as follows:

Industry: cleaning up heavy metal ions and Ca2+ and Mg2+ ions.

Soap: combines with Ca2+ and Mg2+ ions in hard water to reduce hardness.

Photography: Fe(III)EDTA was used as the oxidant.

Textiles: Combined with heavy metals.

Food: Used as a preservative to prevent oxidation of heavy metals.

Cosmetics: Add EDTA to store cosmetics.

Oil production: Add EDTA to prevent mineral precipitation.

Milk: Clean milk bottles.

Nitrogen oxides: treatment of exhaust gases.

Mercury poisoning treatment: Use the disodium calcium salt of EDTA - disodium calcium ethylenediaminetetraacetate [3] (EDTA Na2-Ca) to treat heavy metal poisoning. It can treat people with mercury poisoning, and it can also treat people with lead poisoning. EDTA uses the property of combining with heavy metals to form stable and soluble salts, which are excreted in the urine.

Dentistry: used to remove some organic or inorganic substances during root canal treatment.

Soda: The vitamin C and sodium benzoate contained in soda may produce the carcinogen benzene, which can be treated with EDTA.

Biochemistry: EDTA is used in molecular biology to prevent the effect of metal ions on enzymes.

Water testing: Test the hardness of water. Application of microplate reader

Microplate reader is mainly used to detect absorbance values ??in enzyme-linked immunosorbent assay reactions. Its purpose is mainly used in the determination of ELISA reagents and is widely used in various laboratories, including clinical experiments. room.

The principle of microplate reader measurement is to detect the absorbance value of the analyte at a specific wavelength.

Detection unit:

When light passes through the object to be detected, the energy difference before and after is the energy absorbed by the object to be detected. At a specific wavelength, the concentration of the same object to be detected is the same as that of the object being detected. The energy absorbed is quantitatively related.

The detection unit is represented by the OD value. OD is the abbreviation of optical delnsity (optical density), which indicates the optical density absorbed by the detected object. OD=log (1/trans), where trans is the optical density of the detected object. Transmittance value. According to the Bouger-amber T-beer rule, the OD value has the following relationship with the light intensity:

E=OD=logΙ0/Ι, where E represents the absorbed optical density, and I0 is the light intensity before the detection object , I is the light intensity coming out of the object to be detected.

The OD value is calculated by the following formula:

E＝OD=C×D×E

C is the concentration of the test substance

D is the thickness of the detected object

E is the molar factor

Measured at a specific wavelength. Each substance has its specific wavelength. At this wavelength, the substance can absorb the most light energy. If you choose other wavelength ranges, the detection results will be inaccurate. Therefore, when measuring the detection object, we select a specific wavelength for detection, which is called the measurement wave length.

However, each substance still has a certain non-specific absorption of light energy. In order to eliminate this non-specific absorption, we select a reference wavelength to eliminate this inaccuracy. At the reference wavelength, the absorption of light from the test object is minimal. The difference in absorbance between the detection wavelength and the reference wavelength eliminates non-specific absorption.

Microplate readers are generally used mainly in rapid detection of food safety drug residues, such as clenbuterol, chloramphenicol, furazolidone, furazolidone, When using ELISA kits for rapid detection of residues such as nitrofurantoin, sulfonamides, fluoroquinolones, streptomycin, and penicillin, a microplate reader is generally used for quantitative detection. If you need to build a laboratory, Wanger Company can provide testing laboratory construction plans and related detailed suggestions. Contact person: Mr. Liu 010-89191492 Email: pzwanger008@163. What are the precautions when using a microplate reader?

1. Working environment The microplate reader is a precision optical instrument, so a good working environment can not only ensure its accuracy and stability, but also extend its service life.

a. The instrument should be placed in a location free from strong magnetic fields and interference voltages.

b. The instrument should be placed in an environment where the noise is less than 40 decibels.

c. To delay the aging of optical components, direct sunlight should be avoided.

d. The operating environment temperature should be between 15℃~40℃, and the ambient humidity should be between 15%~85%.

e. The voltage should remain stable during operation.

f. The air in the operating environment is clean and free of water vapor and smoke.

g. Maintain a dry, clean, level work surface and sufficient operating space.

2. Precautions for using the microplate reader

a. Use a pipette to add liquid, and do not mix pipette tips.

b. The plate must be washed cleanly to avoid cross-contamination.

c. Strictly follow the instructions of the kit and ensure the reaction time is accurate.

d. Do not spill samples or reagents on the surface or inside of the instrument, and be sure to clean it after completing the operation.

e. Do not turn off the power during measurement.

f. For deviations in measurement results caused by kit problems, the parameters should be modified in a timely manner according to the actual situation to achieve the best results.

g. Cover the dust cover after use.

In the event of technical failure, you should contact the manufacturer in time and do not disassemble the microplate reader without authorization. What units are used for microplate readers?

Microplate readers are basic experimental instruments and are the final reading device for ELISA experiments. It can be used in schools, hospital laboratory departments, laboratories, and places like Food and Drug Administration, Industrial and Commercial Bureau, Animal Husbandry Bureau, etc. It is widely used. Domestic brands include Leidu, Hantang, Kehua, etc.

What are the guides for purchasing a microplate reader?

ELISA kit microplates can be divided into 48-well microplates and 96-well microplates according to the number of wells. The commonly used microplate is a 96-well microplate, which should be selected according to your microplate reader. In addition, ELISA plates are divided into detachable ELISA plates and non-detachable ELISA plates. For non-detachable ELISA plates, the strips on the entire board are connected together, and all the holes are connected together; and the detachable enzyme plate is that the strips on the plate are separated. The separated strips are divided into 12-hole well strips and 8-well well strips. The frame of the enzyme plate can be repeated. applied.

ELISA kits generally use detachable enzyme-labeled plates now. If you bought some detachable enzyme-labeled plates before, you can just buy some enzyme-labeled strips in the future. . The microplates made by different manufacturers look similar overall, but some small details will be different, such as structure, etc. This is mainly because they need to be used with different microplate readers, so when you When choosing to purchase a microplate reader, you should also consider the appearance of your microplate reader. However, they are generally adapted, and only individual ones will be different.

Because the material of ELISA kit enzyme plate is generally polystyrene (PS), and polystyrene has poor chemical stability and can be dissolved by a variety of organic solvents (such as aromatic hydrocarbons, halogenated hydrocarbons) etc.), will be corroded by strong acids and alkalis, is not resistant to grease, and will easily change color after being exposed to ultraviolet light, so you must pay attention to these during use. At this time, quartz enzyme plate is the best choice, which can be used repeatedly and is resistant to corrosion by acids, alkalis and organic solvents. What are the applications of radioisotopes in agriculture? Specific points.

Isotope tracing Isotope tracing has been widely used in agricultural production and research. It is mainly used to study the photosynthesis of crops, material transport in the body, fertilizer utilization, pesticide residues and nutritional metabolism of livestock, etc., so as to correctly formulate measures for crop cultivation management and reasonable and effective application of fertilizers and pesticides, and for the feeding and management of livestock. etc. to provide scientific basis. What are the advantages of the application of the Internet in agriculture

Agriculture in the Internet+ era is more obvious in marketing channels and can be applied in the entire production process. There is a word called things Networking means that the Internet can be used to control and monitor the entire crop growth process. I hope it can help you. I hope you will adopt it. I am doing a task. Thank you. What are the main contents of the application of microorganisms in agriculture?

Generally speaking, there are microbial nitrogen fixers, that is, nitrogen-fixing bacteria are used to fix nitrogen in the air for plants to absorb and utilize; microbial pesticides are produced by using certain microorganisms that can specifically parasitize certain insects and certain fungi. Microbial pesticides are produced. At the same time, the secondary metabolites of microorganisms can also be used as pesticides to kill bacteria, such as mucormycin; microbial fermentation is used to decompose organic matter into small molecular substances and release inorganic salts for plants to absorb and utilize. Agriculture It is called retting; microbial feed. The microorganisms themselves have rich nutritional value and are perfect for use as feed.

What are the planting links where IoT technology is applied in agriculture: temperature and humidity monitoring, automated irrigation and ventilation, etc.

Processing links: temperature and humidity monitoring in the cold chain, product traceability system? Anti-counterfeiting system

Sales link: Anti-counterfeiting identification of products, rapid identification, and extended smart refrigerators, etc.

Anti-counterfeiting traceability of seeds, traceability of pigs and cattle, etc.? Projects such as safe meat and safe milk