Is it reasonable for a travel agency to charge a ticketing fee of 1,000 yuan for a business ticket worth 40,000 yuan?

Unreasonable. Because the travel agency's quotation already includes profit, and after the travel agency registers and pays the fee, it is definitely unreasonable for the travel agency to charge an additional handling fee when issuing the ticket. Therefore, it is unreasonable for the travel agency to charge a ticket issuance fee of 1,000 yuan for a business ticket of 40,000 yuan. The fees charged by enterprises (travel agencies) that serve travelers for providing travel services are generally called handling fees, which include the travel agency's reasonable expenses, taxes and profits.