What is the difference between the images observed under ordinary optical microscope, dark field microscope and phase contrast microscope?

First of all, we should distinguish it from the functionality of the microscope. Ordinary optical microscope, dark field microscope and phase contrast microscope belong to three different types of microscopes. Because of their different functions, the observation effect is also different. For example, the dark field microscope mainly uses its illumination mode as the dark field mode, and the observation effect on the observed object is completely different from that of the ordinary optical microscope. This is also the case. Please learn more about the function of microscope.

Ordinary optical microscope, that is, bright field microscope; Dark field microscope, when imaging, will highlight the details of the sample, and the outline is dark. Take thin strip as an example. In the dark field, you will find that the edge of the board is bright and the plane part is dark. The phase contrast microscope adopts a special design, so that the phase difference between the direct light and the side light is a quarter wavelength, and then interference occurs. That is to say, the greater the detail changes, the more serious the coherent cancellation of the light will be, the darker it will be, and you will feel a little stereoscopic effect.