Traditional Culture Encyclopedia - Photography major - American red squid details
American red squid details
Chinese Name: American Red Squid Latin Scientific Name: Humboldt Squid Field: Animal Kingdom: Mollusca: Cephalopoda: Sheath Subclass: Squid Suborder: Ophiopogonidae: Silurus: Silurus: Genus: American Red Squid Classification, Keyword Overview, Medical Value and Classification Name: American Red Squid. American Red Squid English: Humboldt Squid /Jumbo Squid/Jumbo Flying Squid English Scientific Name: Dosidicus gigas: Animalia, Mollusca, Cephalopoda, Coleoptera, Squid. He was caught by the tentacles of this animal in the process of shooting red squid. However, after 30 minutes, the monster released him and Castle escaped. His hard diving suit saved him. American red squid can quickly change its body color from crimson to white by using its own pigment cells. Experts pointed out that this is a way of communication for American red squid. Castle didn't flinch after that crisis. He returned to the water. He and his colleagues fixed a camera on the American red squid to record the mysterious underwater world. From June 5438 to1October 065438, 2007, the TV channel broadcasted their new documentary, which revealed the activity habits and living environment of American red squid and provided vivid information for people to understand American red squid and further study this animal. A new type of chitosan was prepared by using cartilage waste from the food processing of American red squid (Dosidicus Gigas) with medicinal value as raw materials, including crushing, dehydration and degreasing, enzymolysis, deproteinization, alcohol precipitation, ion exchange and desalination. The yield of the final product reached 16 2%. Polysaccharide is degraded by commercial chondroitin sulfate ABC enzyme, dermatanase sulfate, hyaluronidase (from Streptomyces hyaluronate) and rHCLase owned by the laboratory. According to the substrate specificity of the enzyme, the prepared polysaccharide was determined to be CS. The content of new CS in the sample was detected by carbazole reaction, and the content of protein in the sample was detected by BCA protein quantitative method. The results show that the content of new chitosan in the sample is above 97%, and the content of protein is below 2%. 2. Preliminary analysis of the structure of the new CS polysaccharide: According to the dextran molecular weight standard, the average molecular weight of the prepared NER-CS polysaccharide was determined to be about 623.2 kDa by gel filtration HPLC analysis. Through the complete degradation of chondroitin sulfate ABC enzyme and HPLC disaccharide composition analysis, it was found that NER-CS mainly contained [glcaβ1-3 galnac] [glcaβ1-3 galnac (4s)], [GlcAβ 1-3GalNAc(6S)] and E- units. Preliminary analysis of biological activity of 3NER-CS:1) The anticoagulant activity of NER-CS was detected by ligand kit. The results showed that the anti-FIIA and anti-FXa activities of NER-CS were 20±2 U/mg and 0.9±0.2 U/mg, respectively, which were about 65 48 00% of the anti-FIIA activities of the positive control heparin. 2) The antitumor activity of NER CS in mice was analyzed by using the 4T 1 cell metastasis model of breast cancer. The results showed that the inhibitory rates of 50, 100 and 200μg NER-CS were 54.3 2.5%, 75. 1 3.5% and 42.9 2.6544, respectively. 3) Biotinylated NER-CS was immobilized on the CM5 wafer modified by avidin, and the interaction activity of NER-CS with five growth factors and three selectins was analyzed. The results showed that the interaction activity of NER-CS with growth factor HGF and basic fibroblast growth factor was high, and the affinity constants were (3.471.73) x10-6, (5.39) respectively. 4. Preparation of 4.NER oligosaccharide and analysis of its antitumor activity.
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