Urgent! ! Ask a few questions about microphotography! ! !

1. Because the light is dark and the field of view may be small, it is best to have a lens with manual focusing function and variable focal length. At this time, it is necessary to manually adjust the focus point.

2. The exposure of the camera should be set to P mode or manual mode (if the aperture and shutter can be adjusted). Do not use automatic mode. Even if the A mode is used, the flash is useless, but it will cause scattered light, which is very troublesome.

3. If the manual exposure mode is the best, at this time, you can adjust the aperture to a small aperture (f value is large, such as 16 ~ 22) to ensure the depth of field in the microscope, and then use a slow shutter (a fraction of a second to several tens of seconds) to delay the self-portrait exposure, or use a pull wire to release and control the exposure. Never press the shutter directly by hand, otherwise it will shake and blur. During exposure, it is necessary to downshift 1 ~ 1.5 to highlight the position of the fluorescent mark without blurring it due to overexposure.

4. Try it several times, and see what exposure value is needed and the number of demotions is better from the effect. Anyway, digital cameras can watch at any time.