Microbiology experiment book information II

Topic: Microbiology Experiment (2 1 Century University Textbook) (Life Science)

ISBN: 7030 10648

Author: He Shaojiang, Zhao Bin

Press: Science Press

Pricing: 24

Page count: 285

Release date: August 2002-1

Version: 1

Format: 16

Packaging: hardcover

Introduction: Microbiology is a very practical subject, and the experimental technology of microbiology is an important content of this subject. This book introduces: ① Basic experimental operation methods and techniques of microbiology. Including the research methods of microbial cell morphology, pure culture technology of microorganisms, separation, purification and identification methods of microorganisms, etc. ② Operating techniques and methods of microbial physiology, microbial genetics and bacterial serology identification. (3) Microbiology application technology, including microbial cell luminescent enzyme gene labeling, separation of specific microorganisms under different background conditions (such as soil, water, insects, animal and plant materials) and research technology of biological characteristics.

The authors of this book are teachers who have been engaged in microbiology teaching and research for a long time. The experimental methods are standardized, and some latest international experimental techniques are introduced in the book.

This book can be used as a teaching book for life science, microbiology, biotechnology, animal engineering, resources and environment, and plant pathology in colleges and universities, and can also be used as a reference book for relevant scientific and technological personnel.

Directory:

The first part is the research method of microbial morphology.

A, microscope technology

A, ordinary optical microscope

Second, the dark field microscope

Third, the fluorescence microscope

Fourth, the phase contrast microscope

Verb (abbreviation of verb) electron microscope

Six, microphotography

Morphological observation of class ⅱ microorganisms

1. Morphological observation of bacteria

Experiment 1 Simple staining and Gram staining of bacteria

Experiment 2 Capsule staining of bacteria

Experiment 3 Bacterial staining of bacteria

Experiment 4 Differential staining method of Bacillus thuringiensis cells and crystals

Experiment 5 bacterial flagella staining (observation of bacterial activity)

Culture and observation of six experimental cyanobacteria

2. Morphological observation of actinomycetes

In experiment 7, the morphology of actinomycetes was observed by dialysis culture (including insertion culture) with transparent adhesive paper agar plate.

Experiment 8: Printing and dyeing methods of actinomycetes

Three. Morphological observation of fungi

Preparation and observation of nine kinds of mold immersion specimens

Experiment/preparation of 10/0 mold seal sample

Experiment 1 1 Culture and observation of mold-bound spores

Experiment/culture and observation of yeast ascospores in kloc-0/2

Observation of mushroom fruiting body by experiment 13 tablet method

Experiment 14 Culture and Observation of Conidia of Fusarium

Four. Morphological observation of virus

Experiment 15 Staining and Observation of Insect Virus Polyhedron

Experiment/Observation of Kloc-0/6 Plaque Culture

Verb (abbreviation of verb) Morphological observation of algae and protozoa

Morphological observation of Chlamydomonas andersonii and Spirogyra

Experiment 18 culture and observation of paramecium and euglena

Experiment 19 culture and observation of amoeba

Ⅲ Determination of microbial size and quantity

Experiment 20 Determination of Microbial Cell Size

Experiment 2 1 Microscopic direct counting method of microbial cells

Experiment 22 dilution plate counting method

Experiment 23 dilution culture counting method (MPN)

In experiment 24, the growth curve of Escherichia coli was determined by turbidimetry.

The second part is pure cultural technology.

Ⅰ medium

First, the source and function of nutrients in the culture medium

Second, the type of culture medium

Third, the preparation method of culture medium

Four, the preparation of several commonly used culture media

Experiment 25 Preparation of beef paste Peptone Medium

Experiment 26 Preparation of High 1 Synthetic Medium

Experiment 27 Preparation of Martin-Bengal Red Medium

Experiment 28 Preparation of Potato Sucrose Agar Medium

Experiment 29 Preparation of Soil Leaching Media

Experiment 30 Preparation of wort medium and rice koji juice medium

Experiment 3/preparation of kloc-0/gelatin medium

Experiment 32 Preparation of litmus milk medium

Second, sterilization and disinfection

First, dry heat sterilization

Second, moist heat sterilization.

Thirdly, filtering and sterilizing.

Fourth, ultraviolet sterilization.

V. Chemical disinfection and sterilization

ⅲ microbial inoculation technology

First, the preparation work before vaccination

Second, the inoculation method

Part III Nutrition and Environmental Conditions of Microorganisms

Experiment 33 Effects of Nutrients on the Growth of Aspergillus Niger

Experiment 34 Effect of Oxygen on Microbial Growth

Experiment 35 Effect of temperature on microbial growth

Effect of Hydrogen Ion Concentration on Microbial Growth in Experiment 36

Experiment 37 Ultraviolet Sterilization Test

Experiment 38 Effects of Chemical Reagents on Microorganisms

Experiment 39 Determination of Salt Tolerance of Microorganisms

The fourth part is the separation, purification and identification of microorganisms.

1. Separation and purification technology for obtaining pure culture of microorganisms

Experiment 40 Isolation and Counting of Aerobic Bacteria in Soil (Line Separation Method)

Experiment 4 1 Isolation of nitrogen-fixing bacteria from soil with selective medium.

Experiment 42 Isolation and Counting of Actinomycetes in Soil

Experiment 43 Isolation of Rare Actinomycetes

Experiment 44 Isolation of Actinomyces Franklin

Experiment 45 Isolation and Counting of Fungi in Soil

Separation of Yeast in Experiment 46 Daqu

Experiment 47 Basidiomycetes Ejection Separation Method

Experiment 48 Isolation of Rhizobia from Leguminosae Nodules

Experiment 49 Isolation of Bacillus thuringiensis from dead insects

Experiment 50 Isolation of methanogenic bacteria from biogas slurry

Experiment 5 1 isolation of phage from natural environment

Experiment 52 Separation of Algae in Soil

Experiment 53 Isolation of Protozoa from Soil

Routine experiment of pure culture identification of class ⅱ bacteria

Identification of representative microbial colonies in Experiment 54

Experiment 55 unique carbon source test

Experiment 56 unique nitrogen source test (inorganic nitrogen)

Experiment 57 Decomposition experiment of carbon source of sugar, alcohol and glycoside

Experiment 58 Starch Hydrolysis Test

Experiment 59 cellulose decomposition test

Experiment 60 Pectin Decomposition Test

Experiment 6 1 oil hydrolysis test

Experiment 62 methyl red (M.R.) test

Experiment 63 Acetylmethyl Methanol (V.P.) Test

Experiment sixty-four citrate test

Experiment 65 Catalase test

Experiment 66 gelatin liquefaction test

Experiment 67 litmus milk test

Experiment 68 Ammonia Production Experiment

Experiment 69 Hydrogen Sulfide Generation Test

Experiment 70 Nitrate Reduction Test

Experiment 7 1 indole test

Experiment 72 phenylalanine dehydrogenase test

Experiment seventy-three lecithin enzyme test

Serological Identification of Type Ⅲ Bacteria

Preparation of antiserum for Experiment 74

Experiment 75 Direct agglutination reaction

Experiment 76 Immunoelectrophoresis

Experiment 77 Two-way immunodiffusion

The fifth part is microbial genetics experiment.

Experiment 78 Preparation and Transformation of Competent Cells

Experiment 79 Extraction of Total DNA from Bacteria

Experiment 80 Extraction of Plasmid DNA

Experiment 8 1 DNA agarose gel electrophoresis

Experiment 82 Recovering DNA from Gel

Experiment 83 Rapid Detection of Plasmid DNA

Experiment 84 RT-PCR

Experiment eighty-five PCR technology (polymerase chain reaction technology)

Experiment 863- Parental hybridization

Experiment 87 Specific Transduction of Bacteria

Experiment 88 Screening of auxotrophic mutants by UV mutagenesis

Experiment 89 Screening Lactose Fermentation Mutants by Nitrite Mutation

Experiment 90 Insertion Mutation Caused by Transposon

Experiment 9 1 bacterial supplementary test

Experiment 92 E.coli Interrupted Hybridization Experiment

Experiment 93 Protoplast Fusion

Experiment 94 Isolation and exchange of Neurospora.

Experiment 95 Preservation of Microbial Species

Part VI Applied Microbiology Experiment

Experiment 96 nodulation test of rhizobia

Experiment 97 Determination of nitrogenase activity in leguminous plant nodules

Experiment 98 Luminase Gene Labeling of Rhizobia and Detection of Labeled Strains

Experiment 99: Vaccinium arbuscular mycorrhiza staining (VAM)

Experiment 1 bioassay of Bacillus thuringiensis insecticide

Experiment 1: determination of antibiotic titer (tube-dish method)

Experiment 1 cultivation and titer determination of Helicoverpa armigera nuclear polyhedrosis virus

Experiment 1-3 alcohol fermentation (with pasteur effect)

Experiment 1 Tetralactic Acid Fermentation

Experiment 15: making yogurt

Experiment 16 cultivation of edible fungi

Experiment 17 Detection of Total Bacteria and Coliform Group in Water

Experiment 18 Detection of Total Colony and Coliform Group in Food

Experiment 19 detection of microorganisms in the air

Experiment 1 Immobilization Technology of Yeast Cells

Appendix 1 Cleaning Methods of Common Glassware in Microbiological Experiment Class

Appendix II Strains Commonly Used in Teaching

Appendix III Formula of Common Medium

Appendix IV Preparation of Common Dyeing Solutions

Appendix V Preparation of Common Buffer

Appendix VI Preparation of Common Reagents and Indicators

Appendix VII Microbial Turbidity Counting Method: Preparation of Mailan Turbidimeter and Comparison Table of Bacteria Counting

Appendix VIII Names and Conversion of Several Legal Units

Appendix IX Statistical Table of Maximum Probability

Main references