What is the reason for the low content of Coomassie Bright Method?

The concentration of interferents is very high. Potassium, sodium, magnesium ions, Tris buffer, sugar and sucrose, glycerol, mercaptoethanol, EDTA, etc. , thus interfering with Coomassie bright method, will also interfere with Coomassie bright method. When the concentration of interferents is high, the content of free dyes will be greatly reduced, which will lead to the nonlinearity of the standard curve and make the display results show less content.