20 13 smog weather statistics

(1) Treating lung tissue with trypsin can decompose glycoprotein on the surface of liver cells and lose related substances between cells, thus obtaining cell suspension.

(2) Adding appropriate lung cell culture medium to provide nutritional conditions for cell proliferation.

(3) Take a number of animal cell culture bottles and divide them into 4 groups, with 3 bottles in each group, and add the same amount of cigarette physiological saline extract and different concentrations of physiological saline, and the group added with physiological saline is the control group.

(4) Put the animal cell culture bottle in a CO2 incubator, and after a period of culture, observe the morphological changes of cells and count the proportion of abnormal cells to normal cells.

(5) Predicting experimental data and designing tables. (See the answer for details)

So the answer is:

(1) trypsin

(2) Adding a proper amount of lung cell culture solution.

(3) Take a number of animal cell culture bottles, divide them into 4 groups, 3 bottles in each group, and add the same amount of cigarette physiological saline extract and different concentrations of physiological saline respectively.

(4)CO2 incubator? Observe the morphological changes of cells and count the proportion of abnormal cells in normal cells.

(5)