Brief introduction of autoradiography

Directory 1 Note: This is the redirection portal, and * * * enjoys the contents of autoradiography. For the convenience of reading, autoradiography in the following text has been automatically replaced by autoradiography. You can click here to restore the original appearance, or you can use the remarks to show 1 to mark autoradiography, which is also known as autoradiography. It is a cytochemical and histochemical method, which uses photographic film or emulsion to observe the uptake of radioactive substances in organisms, so as to determine the distribution, transfer and metabolism of substances in organisms. That is to say, compressed specimens and sliced specimens or living bodies that have ingested specific radioactive substances are placed in a dark room and closely contacted with photographic emulsion. The part exposed by the radiation emitted by radioactive substances ingested in the living body shows black silver particles after development. According to different observation levels, it can be divided into macroscopic self-imaging (visible light self-imaging), microscopic self-imaging (self-imaging displayed by optical microscope) and ultramicroscopic self-imaging (self-imaging displayed by electron microscope). Macroscopic autoradiography is not only used to check the distribution of substances in tissue slices, but also used to determine trace substances together with paper chromatography and electrophoresis. The radionuclides used are 13 1I, 32P, 35S, 90Sr, 59Fe, 45Ca and14c; Autoradiography by naked eye. Microscopic and ultramicro autoradiography mainly uses 14C and 3H.